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Home / Primary Antibodies

SF1 Antibody / Splicing factor 1 (RQ6729)

SKU : BHA17119142

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NSJ Bioreagents

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Mfr.No.	RQ6729
Antigen	SF1
Cellular Localization	Nuclear
Clonality	Polyclonal (rabbit origin)
Description	Splicing factor 1 also known as zinc finger protein 162 (ZFM162) is a protein that in humans is encoded by the SF1 gene. This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.
Host	Rabbit
Immunogen Region	Recombinant human protein (amino acids R160-Q266) was used as the immunogen for the Splicing factor 1 antibody.
Isotype	Rabbit IgG
Reactivity	Human, Mouse, Rat
Recombinant	No

Uniprot	Q15637

Buffer	Lyophilized from 1X PBS with 2% Trehalose
Concentration	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format	Antigen affinity purified
Purification	Antigen affinity purified
Storage	After reconstitution, the Splicing factor 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Applications	WB, IHC-P, IF, FACS
Dilution	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells

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SKU	Supplier No.	Size	Your price	Quantity
BHA17119142	RQ6729	100ug	$419		Add to Cart

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