S100A4/FSP1 (Fibroblast specific protein 1) belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. FSP1 exerts its function via direct interaction with a number of proteins including p53, p63, non-muscle myosin IIA, a6b4 integrin, and liprin b1. FSP1 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A recombinant human partial protein (amino acids 1-200) was used as the immunogen for the recombinant FSP1 antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human, Mouse
Note
The optimal dilution of the FSP1 antibody for each application should be determined by the researcher.
Uniprot
P26447
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the FSP1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, FACS, IF, IHC-P
Description
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT