Required for rRNA maturation and formation of the 60S ribosomal subunits. This protein binds 5S RNA.Frigerio J.-M., Biochim. Biophys. Acta 1262:64-68(1995).Gregory S.G., Nature 441:315-321(2006). Odintsova T.I., J. Protein Chem. 22:249-258(2003).
Host
Rabbit
Immunogen
Synthesized peptide derived from internal of Human RPL5.
Involvement In Disease
Diamond-Blackfan anemia 6 (DBA6)
Raised In
Rabbit
Reactivity
Human, Mouse, Rat
Regulatory
RUO
Relevance
Required for rRNA maturation and formation of the 60S ribosomal subunits. This protein binds 5S RNA.
Frigerio J.-M., Biochim. Biophys. Acta 1262:64-68(1995). Gregory S.G., Nature 441:315-321(2006). Odintsova T.I., J. Protein Chem. 22:249-258(2003).
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous levels of total RPL5 protein.
Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs
Protein Families
Universal ribosomal protein uL18 family
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.