Mouse Anti-BoVIne Rhodopsin Monoclonal IgG1

Mfr.No.	SMC-176, SMC-176D, SMC-176C, SMC-176D-A390, SMC-176D-A488, SMC-176D-A594, SMC-176D-ALP, SMC-176D-APC, SMC-176D-BI, SMC-176D-FITC, SMC-176D-HRP, SMC-176D-PCP, SMC-176D-RPE, SMC-176S
Immunogen Species	Bovine
Alternative Names	OPN2 Antibody, opsd Antibody, opsin 2 Antibody, opsin 2 rod pigment Antibody, opsin2 Antibody, RHO Antibody, RP4 Antibody, MGC138309 Antibody, Retinitis Pigmentosa 4 Antibody
Antigen	Rhodopsin
Categories	Antibodies
Cellular Localization	Membrane
Certificate Of Analysis	1 ug/ml of SMC-176 was sufficient for detection of rhodopsin in 10 ug of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Clonality	Monoclonal
Conjugate	Unconjugated, ATTO 390, ATTO 488, ATTO 594, Alkaline Phosphatase, APC, Biotin, FITC, HRP, PerCP, RPE
Description	Mouse Anti-Bovine Rhodopsin Monoclonal IgG1
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Bovine Rhodopsin, Bos Taurus (Cattle)
Immunogen Region	Bovine Rhodopsin
Isotype	IgG1
Launch Date	2010/06/29
Net Weight (g)	0.1
Reactivity	Mammals, Anchovies, Amphibians, Extinct Spiny Shark, Fish, Avian, Shark
Recombinant	FALSE
Regulatory	RUO
Species	Bos Taurus (Cattle)
Species Reactivity Medium	Mammals, Fish, Avian, Amphibians, Shark

Swissprot	P02699
Accession Number	NP_001014890.1
Gene Id	509933
Target	Rhodopsin
Clone No	4D2
Research Area	Neurotransmitter Receptors, Cell Signaling, Rhodopsins, Neuroscience

Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000), IHC (1000); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~40kDa. Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	ELISA, IHC, IP, WB, ICC/IF
Description	WB ; IHC ; ICC ; IF ; IP ; ELISA
Dilution	WB (1:1000), IHC (1000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Molday R.S., Hicks D., and Molday L. (1987) Invest Ophthalmol Vis Sci. 28: 50-61. 2. Ridge K.D., Lee S.S.J., and Abdulaev N.G. (1996) J of Biol Chem. 271: 7860-7867. 3. Matsuyama T., Yamashita T., Imai H. and Shichida Y. (2009) J Biol Chem. Manuscript M109.063875. 4. Feurstein S.E., et al. (2009) Biochemistry. 48(45): 10733-10742. 5. Iannaccone A., et al. (2006) Vision Res. 46(27): 4556-4567.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11901175-1	SMC-176D	Unconjugated	100 ug	$330	Add to Cart
BHA11901175-2	SMC-176C	Unconjugated	25 ug	$179	Add to Cart
BHA11901175-3	SMC-176D-A390	ATTO 390	100 ug	$384	Add to Cart
BHA11901175-4	SMC-176D-A488	ATTO 488	100 ug	$383	Add to Cart
BHA11901175-5	SMC-176D-A594	ATTO 594	100 ug	$383	Add to Cart
BHA11901175-6	SMC-176D-ALP	Alkaline Phosphatase	100 ug	$376	Add to Cart
BHA11901175-7	SMC-176D-APC	APC	100 ug	$382	Add to Cart
BHA11901175-8	SMC-176D-BI	Biotin	100 ug	$378	Add to Cart
BHA11901175-9	SMC-176D-FITC	FITC	100 ug	$374	Add to Cart
BHA11901175-10	SMC-176D-HRP	HRP	100 ug	$370	Add to Cart
BHA11901175-11	SMC-176D-PCP	PerCP	100 ug	$382	Add to Cart
BHA11901175-12	SMC-176D-RPE	RPE	100 ug	$380	Add to Cart
BHA11901175-13	SMC-176S	Unconjugated	12 ug	$44	Add to Cart

Rhodopsin Antibody, Clone 4D2 - SMC-176