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Home / Antibodies

Retinoblastoma Antibody / Rb1 (V3578)

SKU : BHA17114269

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NSJ Bioreagents

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Mfr.No.	V3578, V3578-20UG, V3578-100UG
Antigen	Retinoblastoma
Clonality	Monoclonal
Description	Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.
Formulation	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Host	Rabbit
Immunogen Region	A human recombinant partial protein was used as immunogen for this recombinant RB1 antibody.
Isotype	IgG,kappa
Predicted Reactivity	Human, Mouse
Reactivity	Human, Mouse
Recombinant	Yes
Subcellular Location	Nuclear

Uniprot	P06400
Clone No	RB1/2313R

Concentration	0.2 mg/ml
Format	Purified
Purification	Protein A affinity chromatography
Storage	The recombinant RB1 antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Applications	IHC-P
Dilution	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Data Sheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17114269-1	V3578-20UG	20ug	$219		Add to Cart
BHA17114269-2	V3578-100UG	100ug	$499		Add to Cart

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