Guanine nucleotide-binding protein subunit beta-2-like 1, also known as RACK1, is a 35kDa protein that in humans is encoded by the GNB2L1 gene. It is mapped to 5q35.3. It is a major component of translating ribosomes, which harbor significant amounts of PKC. This gene can provide a physical and functional link between <a href=../search_result.php?search_txt=pkc>PKC</a> signaling and ribosome activation. GNB2L1/RACK1 is also a mediator of agonist-induced Ca(2+) release. Additionally, it is an essential component of an oxygen-independent mechanism for regulating <a href=../search_result.php?search_txt=hif1a>HIF1A</a> stability. Overexpression of RACK1 and PKC-alpha suppressed <a href=../search_result.php?search_txt=clock>CLOCK</a>-BMAL1 transcriptional activity, and it stimulated phosphorylation of BMAL1 by PKC-alpha in vitro.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Human partial recombinant protein (AA 2-317) was used as the immunogen for this RACK1 antibody.
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Human, Mouse, Rat
Recombinant
No
Gene Id
10399
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the RACK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, IF, FACS
Dilution
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells