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Home / Primary Antibodies / Polyclonal Antibodies

Rab5 Antibody - SPC-168

BHA11906942
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[email protected]
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Swissprot P20339
Accession Number NP_004153.2
Gene Id 5868
Target Rab5
Research Area Vesicle Transport| Neuroscience, Cell Signaling, Tags and Cell Markers, Organelle Markers, Cell Markers, Neuron Markers, Presynaptic Markers, Protein Trafficking
Buffer PBS, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Dilutions WB (1:1000), IHC (1:100), ICC/IF (1:80); optimal dilutions for assays should be determined by the user.
Purification Protein A Purified
Scientific Background Rab5 is a 24kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper variable COHO-terminal domains with a cysteine motif implicated in subcellular targeting. Post-translational modification of the cysteine motif with one or two geranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins(3). Each Rab shows a characteristic subcellular distribution (4).
In particular, Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane. It regulates the fusion between endocytic vesicles and early endosomes, as well as the homotypic fusion between early endosomes (5). Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and EEA1 (6). Anti-Rab5 may be used as an early endosome marker.
Shipping Temperature Blue Ice or 4°C
Specificity Detects ~26kDa.
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature -20°C
Applications IHC, WB, ICC/IF
Description WB ; IHC ; ICC ; IF
Dilution WB (1:1000), IHC (1:100), ICC/IF (1:80); optimal dilutions for assays should be determined by the user.
Product Manual SDS
SDS
Reference 1. Stenmark H., and Olkkonen V.M. (2001) Genome Biol. 2: 3007.1-3007.7.

2. Takai Y., et al. (2001) Physiol. Rev. 8:, 153-208.

3. Ali B.R., et al. (2004) J. Cell Sci. 117: 6401-6412.

4. Zerial M., and McBride H. (2001) Nat. Rev. Mol. Cell Biol. 2: 107-117.

5. Sonnichsen B., et al. (2000) J. Cell Biol. 149: 901-913

6. Woodman P.G. (2000) Traffic. 1: 695-701.
Combination SKU Supplier No. Conjugate Size Your price Quantity
BHA11906942-1 SPC-168D Unconjugated 100 ug $264
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BHA11906942-2 SPC-168C Unconjugated 25 ug $125
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BHA11906942-3 SPC-168D-A390 ATTO 390 100 ug $318
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BHA11906942-4 SPC-168D-A488 ATTO 488 100 ug $317
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BHA11906942-5 SPC-168D-A594 ATTO 594 100 ug $317
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BHA11906942-6 SPC-168D-ALP Alkaline Phosphatase 100 ug $310
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BHA11906942-7 SPC-168D-APC APC 100 ug $316
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BHA11906942-8 SPC-168D-BI Biotin 100 ug $312
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BHA11906942-9 SPC-168D-FITC FITC 100 ug $307
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BHA11906942-10 SPC-168D-HRP HRP 100 ug $303
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BHA11906942-11 SPC-168D-PCP PerCP 100 ug $316
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BHA11906942-12 SPC-168D-RPE RPE 100 ug $314
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BHA11906942-13 SPC-168S Unconjugated 12 ug $44
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