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Home / Primary Antibodies

Pyruvate Kinase Antibody / PKM2 (RQ6751)

SKU : BHA17119164

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NSJ Bioreagents

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Mfr.No.	RQ6751
Antigen	Pyruvate Kinase
Cellular Localization	nuclear, Cytoplasmic
Clonality	Polyclonal (rabbit origin)
Description	PKM (Pyruvate Kinase, Muscle), also known as OIP-3, PK3 and PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate (Fru-1, 6-P2). By in situ hybridization, Popescu and Cheng (1990) mapped the THBP1 gene to 15q24-q25. Ashizawa et al. (1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al. (2008) observed that PKM2 binds directly and selectively to tyrosine-phosphorylated peptides.
Host	Rabbit
Immunogen Region	N-terminal region amino acids KDPVQEAWAEDVDLRVNFAMNVGKAR from the human protein were used as the immunogen for the Pyruvate Kinase antibody.
Isotype	Rabbit IgG
Reactivity	Human
Recombinant	No

Uniprot	P14618

Buffer	Lyophilized from 1X PBS with 2% Trehalose
Concentration	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format	Antigen affinity purified
Purification	Antigen affinity purified
Storage	After reconstitution, the Pyruvate Kinase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Applications	WB, IHC-P, IF, FACS
Dilution	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells

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SKU	Supplier No.	Size	Your price	Quantity
BHA17119164	RQ6751	100ug	$419		Add to Cart

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