DLG4 (Discs large homolog 4), also known as Post-synaptic density protein 95 (PSD-95) or SAP-90, is a protein that in humans is encoded by the DLG4 gene. It is a member of the membrane-associated guanylate kinase (MAGUK) family. DLG4 can heteromultimerize with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Overexpression of DLG4 in hippocampal neurons could drive maturation of glutamatergic synapses. DLG4 can orchestrate synaptic development and it has a role in synapse stabilization and plasticity. Ubiquitination of DLG4 through an MDM2-mediated pathway can regulate AMPA receptor surface expression during synaptic plasticity.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
An amino acid sequence from the C-terminus of human PSD-95 (RRVERREWSRLKAKDW) was used as the immunogen for this PSD-95 antibody (100% homologous in human, mouse and rat).
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Human, Mouse, Rat
Recombinant
No
Uniprot
P78352
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the PSD-95 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, IHC-P
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml