This antibody recognizes a protein of 30kDa which is identified as Prohibitin (PHB), an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although PHB mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. It is an ideal mitochondrial marker. It shows anti-proliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A recombinant human partial protein corresponding to amino acids 167-261 was used as the immunogen for the Prohibitin antibody.
Isotype
Mouse IgG2b, kappa
Species Reactivity
Human
Note
Optimal dilution of the antibody should be determined by the researcher.
Uniprot
P35232
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the Prohibitin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, IHC-P
Description
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT