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Home / Antibodies

PRKDC (Ab-2609) Antibody

SKU : BHA10557463

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA178115
Alternative Names	DNPK1; PRKD; PRKDC; XRCC7; P460
Categories	Primary Antibodies
Clonality	polyclonal
Description	The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic propertiesChan DW, et al. (2002) Genes Dev. Sep 15; 16(18): 2333-2338Ding Q, et al. (2003) Mol Cell Biol. Aug; 23(16): 5836-5848Douglas P, et al. (2002) Biochem J. Nov 15; 368(Pt 1): 243-251
Host	Rabbit
Immunogen	Peptide sequence around aa.2607~2611 (V-E-T-Q-A) derived from Human DNA-PK.
Involvement In Disease	Immunodeficiency 26 with or without neurologic abnormalities (IMD26)
Raised In	Rabbit
Reactivity	Human
Regulatory	RUO
Relevance	The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties Chan DW, et al. (2002) Genes Dev. Sep 15; 16(18): 2333-2338 Ding Q, et al. (2003) Mol Cell Biol. Aug; 23(16): 5836-5848 Douglas P, et al. (2002) Biochem J. Nov 15; 368(Pt 1): 243-251
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of total DNA PKcs protein.
Subcellular Location	Nucleus, Nucleus, nucleolus

Uniprot	P78527
Database Links	HGNC:9413 OMIM:600899 KEGG:hsa:5591 STRING:9606.ENSP00000313420 UniGene:Hs.491682
Function	Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect mechanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.
Pathway	Cell cycle
Protein Families	PI3/PI4-kinase family

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Purity	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB

Description

ELISA ; WB, WB ; ELISA

Dilution

WB	1:500-1:1000

Tested Application

ELISA,WB;WB:1:500-1:1000

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10557463	CSB-PA178115	100ul	$297		Add to Cart

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