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Home / Antibodies

POU5F1 Antibody

SKU : BHA10557513

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA694841
Alternative Names	Otc3; OTF3; OTF4; OTF-3;
Categories	Primary Antibodies
Clonality	polyclonal
Description	Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency Pesce, M. and Scholer, H.R. (2001) Stem Cells 19, 271-278. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9. Cauffman, G. et al. (2006) Stem Cells 24, 2685-91.
Host	Rabbit
Immunogen	Peptide sequence around aa.232~236 (R-K-R-T-S) derived from OCT-4
Raised In	Rabbit
Reactivity	Human
Regulatory	RUO
Relevance	Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency Pesce, M. and Scholer, H.R. (2001) Stem Cells 19, 271-278. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9. Cauffman, G. et al. (2006) Stem Cells 24, 2685-91.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of total OCT-4 protein.
Subcellular Location	Cytoplasm, Nucleus

Uniprot	Q01860
Database Links	HGNC:9221 OMIM:164177 KEGG:hsa:5460 STRING:9606.ENSP00000259915 UniGene:Hs.249184
Function	Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Pathway	Signaling pathways regulating pluripotency of stem cells
Protein Families	POU transcription factor family, Class-5 subfamily
Tissue Specificity	Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Purity	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB

Description

ELISA ; WB, WB ; ELISA

Dilution

WB	1:500-1:1000

Tested Application

ELISA,WB;WB:1:500-1:1000

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10557513	CSB-PA694841	100ul	$297		Add to Cart

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