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Home / Primary Antibodies

POLR2A Antibody / RNA polymerase II subunit RPB1 (V7452)

SKU : BHA17116275

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NSJ Bioreagents

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Mfr.No.	V7452, V7452-20UG, V7452-100UG
Clonality	Monoclonal
Description	RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.
Formulation	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Host	Mouse
Immunogen Region	The immunogen for this POLR2A antibody was 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-S5.
Isotype	IgG1,kappa
Predicted Reactivity	Human, Mouse, Rat
Reactivity	Human, Mouse, Rat
Recombinant	No
Subcellular Location	Nuclear

Uniprot	P24928
Clone No	CTD 4H8

Concentration	0.2 mg/ml
Format	Purified
Purification	Protein G affinity chromatography
Storage	Store the POLR2A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Applications	FACS, IF, WB, IHC-P
Dilution	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Weste blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Data Sheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17116275-1	V7452-20UG	20ug	$219		Add to Cart
BHA17116275-2	V7452-100UG	100ug	$499		Add to Cart

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