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Home / Antibodies

POLR2A Antibody / RNA polymerase II subunit RPB1 / Phosphorylated Serine 5 (V7525)

SKU : BHA17116450

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NSJ Bioreagents

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Mfr.No.	V7525, V7525-20UG, V7525-100UG
Antigen	POLR2A
Clonality	Monoclonal
Description	RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.
Formulation	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Host	Mouse
Immunogen Region	The immunogen for this phospho-RNA polymerase II antibody was 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-S5.
Isotype	IgM,kappa
Predicted Reactivity	Human
Reactivity	Human
Recombinant	No
Subcellular Location	Nuclear

Uniprot	P24928
Clone No	CTD 8A7

Concentration	0.2 mg/ml
Format	Purified
Purification	PEG precipitation
Storage	Store the phospho-RNA polymerase II antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Applications	FACS, IF, WB, IHC-P
Dilution	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Data Sheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17116450-1	V7525-20UG	20ug	$219		Add to Cart
BHA17116450-2	V7525-100UG	100ug	$499		Add to Cart

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