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Home / Antibodies

PMEL17 / Melanoma gp100 Antibody (V2856SAF)

SKU : BHA17124424

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NSJ Bioreagents

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Mfr.No.	V2856SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	Cytotoxic T lymphocytes (CTL's) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein.The gp100 molecule is a 100kDa glycosylated protein that is cleaved into a small (26kDa) carboxy-terminal fragment and a larger amino- terminal section (60-64 kDa), which is subsequently cleaved to generate 26kDa and 34-38kDa fragments.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Recombinant human protein was used as the immunogen for the PMEL17 antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human
Note	Optimal dilution of the PMEL17 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P40967

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the PMEL17 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	WB, IHC-P
Description	Weste blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1) (2)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124424	V2856SAF-100UG	100 ug	$499		Add to Cart

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