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Home / Primary Antibodies

Placental Alkaline Phosphatase Antibody / ALPP (V3993)

SKU : BHA17115198

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NSJ Bioreagents

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Mfr.No.	V3993, V3993-20UG, V3993-100UG
Antigen	Placental Alkaline Phosphatase
Clonality	Monoclonal
Description	PLAP is a tissue specific, trophoblast-derived, 70kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+ metallo-glycoprotein that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific AP's in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous. Non-tissue specific AP is found in kidney, liver and bone. This mAb binds equally well to all common allelic variants (S, F, FS and I) of PLAP and to some variants of AP from normal human testis. This mAb can be used as tracer antibody in ELISA to detect PLAP in serum of S, F, FS and I phenotypes.
Formulation	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Host	Rabbit
Immunogen Region	Recombinant full-length human protein was used as the immunogen for the recombinant Placental Alkaline Phosphatase antibody.
Isotype	IgG,kappa
Predicted Reactivity	Human
Reactivity	Human
Recombinant	Yes
Subcellular Location	Cytoplasmic and cell surface

Uniprot	P05187
Clone No	ALPP/2899R

Concentration	0.2 mg/ml
Format	Purified
Purification	Protein A affinity chromatography
Storage	Store the recombinant Placental Alkaline Phosphatase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Applications	WB, IHC-P
Dilution	Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Data Sheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17115198-1	V3993-20UG	20ug	$219		Add to Cart
BHA17115198-2	V3993-100UG	100ug	$499		Add to Cart

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