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Home / Primary Antibodies

Placental Alkaline Phosphatase Antibody / ALPP (V2515IHC)

SKU : BHA17123926

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NSJ Bioreagents

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Mfr.No.	V2515IHC-7ML
Clonality	Monoclonal (mouse origin)
Description	Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.
Formulation	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only
Host	Mouse
Immunogen Region	Recombinant full-length human ALPP/placental alkaline phosphatase protein was used as the immunogen for the PLAP antibody.
Isotype	Mouse IgG2b, kappa
Species Reactivity	Human
Note	Optimal dilution of the PLAP antibody should be determined by the researcher.1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P05187

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the PLAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17123926	V2515IHC-7ML	7 ml	$499		Add to Cart

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