Mouse Anti-Mouse Piccolo Monoclonal IgG2b Kappa

Mfr.No.	SMC-188, SMC-188D, SMC-188C, SMC-188D-A390, SMC-188D-A488, SMC-188D-A594, SMC-188D-ALP, SMC-188D-APC, SMC-188D-BI, SMC-188D-FITC, SMC-188D-HRP, SMC-188D-PCP, SMC-188D-RPE, SMC-188S
Immunogen Species	Human
Alternative Names	ACZ Antibody, Aczonin Antibody, PCLO Antibody, Pico Antibody, presynaptic cytomatrix protein Antibody
Categories	Antibodies
Cellular Localization	Cell Junction, Synapse
Certificate Of Analysis	1 ug/ml of SMC-188 was sufficient for detection of Piccolo in 20 ug of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary antibody.
Clonality	Monoclonal
Description	Mouse Anti-Human Piccolo Monoclonal IgG2b Kappa
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Full length recombinant protein (GenBank ID: BC001304.1), Mus Musculus (Mouse)
Immunogen Region	Human full length recombinant protein (GenBank ID: BC001304.1)
Isotype	IgG2b Kappa
Launch Date	2010/11/04
Net Weight (g)	0.1
Reactivity	Rat, Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species	Mus Musculus (Mouse)
Species Reactivity Medium	Mouse, Rat, Human

Swissprot	Q9QYX7
Accession Number	NP_001104266.1
Gene Id	26875
Target	Piccolo
Clone No	6H9-B6
Research Area	Cell Markers, Neuron Markers, Presynaptic Markers, Neuroscience

Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).
Shipping Temperature	Blue Ice or 4°C
Specificity	Detects ~550kDa. No cross-reactivity to Bassoon.
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	ELISA, WB, ICC/IF
Description	WB ; ICC ; IF ; ELISA
Dilution	WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619. 2. Wang X., et al. (1999) J Cell Biol. 147(1): 151-162. 3. Dresbach T., et al. (2006) J Biol Chem. 281(9):6038-6047.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11901395-1	SMC-188D	Unconjugated	100 ug	$323	Add to Cart
BHA11901395-2	SMC-188C	Unconjugated	25 ug	$179	Add to Cart
BHA11901395-3	SMC-188D-A390	ATTO 390	100 ug	$377	Add to Cart
BHA11901395-4	SMC-188D-A488	ATTO 488	100 ug	$376	Add to Cart
BHA11901395-5	SMC-188D-A594	ATTO 594	100 ug	$376	Add to Cart
BHA11901395-6	SMC-188D-ALP	Alkaline Phosphatase	100 ug	$370	Add to Cart
BHA11901395-7	SMC-188D-APC	APC	100 ug	$375	Add to Cart
BHA11901395-8	SMC-188D-BI	Biotin	100 ug	$372	Add to Cart
BHA11901395-9	SMC-188D-FITC	FITC	100 ug	$367	Add to Cart
BHA11901395-10	SMC-188D-HRP	HRP	100 ug	$362	Add to Cart
BHA11901395-11	SMC-188D-PCP	PerCP	100 ug	$375	Add to Cart
BHA11901395-12	SMC-188D-RPE	RPE	100 ug	$373	Add to Cart
BHA11901395-13	SMC-188S	Unconjugated	12 ug	$44	Add to Cart

Piccolo Antibody, Clone 6H9-B6 - SMC-188