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Home / Antibodies

Phospho-VIM (Ser56) Antibody

SKU : BHA10560104

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA084867
Alternative Names	VIME; vimentin;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.Matsuyama M, et al. (2013)J Biol Chem 288, 35626-35. Haolong C, et al. (2013)PLoS One 8, e73900. Kotula E, et al. (2013)PLoS One 8, e80313.
Host	Rabbit
Immunogen	Peptide sequence around phosphorylation site of serine 56 (A-S-S(p)-P-G) derived from Human Vimentin.
Involvement In Disease	Cataract 30, multiple types (CTRCT30)
Raised In	Rabbit
Reactivity	Human
Regulatory	RUO
Relevance	Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Matsuyama M, et al. (2013)J Biol Chem 288, 35626-35. Haolong C, et al. (2013)PLoS One 8, e73900. Kotula E, et al. (2013)PLoS One 8, e80313.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of Vimentin only when phosphorylated at serine 56.
Subcellular Location	Cytoplasm

Uniprot	P08670
Gene Id	7431
Database Links	HGNC:12692 OMIM:116300 KEGG:hsa:7431 STRING:9606.ENSP00000224237 UniGene:Hs.455493
Function	Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Protein Families	Intermediate filament family
Tissue Specificity	Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Weight	53,652 Da

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB

Description

ELISA ; WB, WB ; ELISA

Dilution

WB	1:500-1:1000

Tested Application

ELISA,WB;WB:1:500-1:1000

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10560104	CSB-PA084867	100ul	$360		Add to Cart

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