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Home / Primary Antibodies

Phospho-TUBA1A/TUBA1B/TUBA1C/TUBA3C/TUBA3E/TUBA4A (Tyr272) Antibody

SKU : BHA10560247

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA289123
Alternative Names	TBA1; TBA1A; TBA4A; TUBA1;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. Adler A.J., Bioorg. Med. Chem. 9:1967-1976(2001).Sugano S., Nat. Genet. 36:40-45(2004).Venter J.C., Submitted (JUL-2005).
Host	Rabbit
Immunogen	Peptide sequence around phosphorylation site of tyrosine 272 (A-T-Y(p)-A-P) derived from Human TUBA1/3/4.
Involvement In Disease	Lissencephaly 3 (LIS3)
Raised In	Rabbit
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Relevance	Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. Adler A.J., Bioorg. Med. Chem. 9:1967-1976(2001). Sugano S., Nat. Genet. 36:40-45(2004). Venter J.C., Submitted (JUL-2005).
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous levels of TUBA1/3/4 only when phosphorylated at tyrosine 272.
Subcellular Location	Cytoplasm, cytoskeleton

Uniprot	Q71U36
Database Links	HGNC:20766 OMIM:602529 KEGG:hsa:7846 STRING:9606.ENSP00000301071 UniGene:Hs.654422
Function	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Pathway	Gap junction Apoptosis
Protein Families	Tubulin family
Tissue Specificity	Expressed at a high level in fetal brain.

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB

Description

ELISA ; WB, WB ; ELISA

Dilution

WB	1:500-1:1000

Tested Application

ELISA,WB;WB:1:500-1:1000

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10560247	CSB-PA289123	100ul	$360		Add to Cart

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