Signal transducer and activator of transcription 5B; STA5B; STAT5; transcription factor STAT5B;
Categories
Primary Antibodies ; Phospho Antibodies
Clonality
polyclonal
Description
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. Ross JA, et al. (2010) J Biol Chem 285, 3582-91 Uddin S, et al. (2003) Biochem Biophys Res Commun 308, 325-30Xue HH, et al. (2002) Int Immunol 14, 1263-71
Host
Rabbit
Immunogen
Peptide sequence around phosphorylation site of serine725/730(A-P-S(p)-P-V) derived from Human STAT5A/B.
Raised In
Rabbit
Reactivity
Human, Mouse, Rat
Regulatory
RUO
Relevance
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
Ross JA, et al. (2010) J Biol Chem 285, 3582-91 Uddin S, et al. (2003) Biochem Biophys Res Commun 308, 325-30 Xue HH, et al. (2002) Int Immunol 14, 1263-71
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous level of STAT5A/B only when phosphorylated at serine 725/ serine 730.
In the virgin, found in most tissues except brain and muscle. During lactation, abundantly found in mammary tissue, as well as in other secretory organs such as salivary gland and seminal vesicle.
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Applications
ELISA, WB
Description
ELISA ; WB, WB ; ELISA
Dilution
WB
1:500-1:1000
Tested Application
ELISA,WB;WB:1:500-1:1000
Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Reviews of Phospho-Stat5a/Stat5b (Ser725/730) Antibody