Phospho-SirT1 (Ser47) Antibody

Mfr.No.	AF3474
Alternative Names	75SirT1 ; hSIR2 ; hSIRT1 ; HST2, S. cerevisiae, homolog of ; NAD dependent deacetylase sirtuin 1 ; NAD dependent protein deacetylase sirtuin 1 ; OTTHUMP00000198111 ; OTTHUMP00000198112 ; Regulatory protein SIR2 homolog 1 ; SIR1_HUMAN ; SIR2 ; SIR2 like 1 ; SIR2 like protein 1 ; SIR2, S.cerevisiae, homolog-like 1 ; SIR2-like protein 1 ; SIR2ALPHA ; SIR2L1 ; Sirt1 ; SirtT1 75 kDa fragment ; Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) ; Sirtuin 1 ; Sirtuin type 1 ;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA.
Immunogen	A synthesized peptide derived from human SirT1 around the phosphorylation site of Serine 47.
Molecular Weight	Observed Mol.Wt.: 110kDa. Predicted Mol.Wt.: 82kDa.
Reactivity	Human, Mouse
Specificity	Phospho-SirT1 (Ser47) Antibody detects endogenous levels of SirT1 only when phosphorylated at Serine 47.
Subcellular Location	Cytoplasm. Mitochondrion and Nucleus, PML body. Cytoplasm. Nucleus. Recruited to the nuclear bodies via its interaction with PML (PubMed:12006491). Colocalized with APEX1 in the nucleus (PubMed:19934257). May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane (PubMed:15469825). Shuttles between nucleus and cytoplasm (By similarity). Colocalizes in the nucleus with XBP1 isoform 2 (PubMed:20955178).

Uniprot

Q96EB6

Gene Id

23411

Sequence

10	20	30	40	50
MADEAALALQ	PGGSPSAAGA	DREAASSPAG	EPLRKRPRRD	GPGLERSPGE
60	70	80	90	100
PGGAAPEREV	PAAARGCPGA	AAAALWREAE	AEAAAAGGEQ	EAQATAAAGE
110	120	130	140	150
GDNGPGLQGP	SREPPLADNL	YDEDDDDEGE	EEEEAAAAAI	GYRDNLLFGD
160	170	180	190	200
EIITNGFHSC	ESDEEDRASH	ASSSDWTPRP	RIGPYTFVQQ	HLMIGTDPRT
210	220	230	240	250
ILKDLLPETI	PPPELDDMTL	WQIVINILSE	PPKRKKRKDI	NTIEDAVKLL
260	270	280	290	300
QECKKIIVLT	GAGVSVSCGI	PDFRSRDGIY	ARLAVDFPDL	PDPQAMFDIE
310	320	330	340	350
YFRKDPRPFF	KFAKEIYPGQ	FQPSLCHKFI	ALSDKEGKLL	RNYTQNIDTL
360	370	380	390	400
EQVAGIQRII	QCHGSFATAS	CLICKYKVDC	EAVRGDIFNQ	VVPRCPRCPA
410	420	430	440	450
DEPLAIMKPE	IVFFGENLPE	QFHRAMKYDK	DEVDLLIVIG	SSLKVRPVAL
460	470	480	490	500
IPSSIPHEVP	QILINREPLP	HLHFDVELLG	DCDVIINELC	HRLGGEYAKL
510	520	530	540	550
CCNPVKLSEI	TEKPPRTQKE	LAYLSELPPT	PLHVSEDSSS	PERTSPPDSS
560	570	580	590	600
VIVTLLDQAA	KSNDDLDVSE	SKGCMEEKPQ	EVQTSRNVES	IAEQMENPDL
610	620	630	640	650
KNVGSSTGEK	NERTSVAGTV	RKCWPNRVAK	EQISRRLDGN	QYLFLPPNRY
660	670	680	690	700
IFHGAEVYSD	SEDDVLSSSS	CGSNSDSGTC	QSPSLEEPME	DESEIEEFYN
710	720	730	740
GLEDEPDVPE	RAGGAGFGTD	GDDQEAINEA	ISVKQEVTDM	NYPSNKS

Concentration	1mg/ml
Format	Liquid
Purification	The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Storage Condition	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.