Phospho-SirT1 (Ser27) Antibody

Mfr.No.	AF4484
Alternative Names	75SirT1 ; hSIR2 ; hSIRT1 ; HST2, S. cerevisiae, homolog of ; NAD dependent deacetylase sirtuin 1 ; NAD dependent protein deacetylase sirtuin 1 ; OTTHUMP00000198111 ; OTTHUMP00000198112 ; Regulatory protein SIR2 homolog 1 ; SIR1_HUMAN ; SIR2 ; SIR2 like 1 ; SIR2 like protein 1 ; SIR2, S.cerevisiae, homolog-like 1 ; SIR2-like protein 1 ; SIR2ALPHA ; SIR2L1 ; Sirt1 ; SirtT1 75 kDa fragment ; Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) ; Sirtuin 1 ; Sirtuin type 1 ;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Immunogen	A synthesized peptide derived from human SirT1 around the phosphorylation site of Ser27.
Molecular Weight	Observed Mol.Wt.: 120kDa. Predicted Mol.Wt.: 82kDa.
Reactivity	Human
Specificity	Phospho-SirT1 (Ser27) Antibody detects endogenous levels of SirT1 only when phosphorylated at Ser27.
Subcellular Location	Cytoplasm. Mitochondrion and Nucleus, PML body. Cytoplasm. Nucleus. Recruited to the nuclear bodies via its interaction with PML (PubMed:12006491). Colocalized with APEX1 in the nucleus (PubMed:19934257). May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane (PubMed:15469825). Shuttles between nucleus and cytoplasm (By similarity). Colocalizes in the nucleus with XBP1 isoform 2 (PubMed:20955178).

Uniprot

Q96EB6

Gene Id

23411

Sequence

10 20 30 40 50
MADEAALALQ PGGSPSAAGA DREAASSPAG EPLRKRPRRD GPGLERSPGE
60 70 80 90 100
PGGAAPEREV PAAARGCPGA AAAALWREAE AEAAAAGGEQ EAQATAAAGE
110 120 130 140 150
GDNGPGLQGP SREPPLADNL YDEDDDDEGE EEEEAAAAAI GYRDNLLFGD
160 170 180 190 200
EIITNGFHSC ESDEEDRASH ASSSDWTPRP RIGPYTFVQQ HLMIGTDPRT
210 220 230 240 250
ILKDLLPETI PPPELDDMTL WQIVINILSE PPKRKKRKDI NTIEDAVKLL
260 270 280 290 300
QECKKIIVLT GAGVSVSCGI PDFRSRDGIY ARLAVDFPDL PDPQAMFDIE
310 320 330 340 350
YFRKDPRPFF KFAKEIYPGQ FQPSLCHKFI ALSDKEGKLL RNYTQNIDTL
360 370 380 390 400
EQVAGIQRII QCHGSFATAS CLICKYKVDC EAVRGDIFNQ VVPRCPRCPA
410 420 430 440 450
DEPLAIMKPE IVFFGENLPE QFHRAMKYDK DEVDLLIVIG SSLKVRPVAL
460 470 480 490 500
IPSSIPHEVP QILINREPLP HLHFDVELLG DCDVIINELC HRLGGEYAKL
510 520 530 540 550
CCNPVKLSEI TEKPPRTQKE LAYLSELPPT PLHVSEDSSS PERTSPPDSS
560 570 580 590 600
VIVTLLDQAA KSNDDLDVSE SKGCMEEKPQ EVQTSRNVES IAEQMENPDL
610 620 630 640 650
KNVGSSTGEK NERTSVAGTV RKCWPNRVAK EQISRRLDGN QYLFLPPNRY
660 670 680 690 700
IFHGAEVYSD SEDDVLSSSS CGSNSDSGTC QSPSLEEPME DESEIEEFYN
710 720 730 740
GLEDEPDVPE RAGGAGFGTD GDDQEAINEA ISVKQEVTDM NYPSNKS

Concentration	1mg/ml
Format	Liquid
Purification	The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Storage Condition	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Applications	ELISA, WB
Dilution	WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Manual
MSDS
Sample Preparation For Western Blotting.
Western Blotting Handbook.

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SKU	Supplier No.	Size	Your price	Quantity
BHA12909766	AF4484	100ul	$350		Add to Cart
BHA12909766	AF4484	200ul	$450		Add to Cart