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Home / Primary Antibodies

Phospho-SHC1 (Tyr349) Antibody

SKU : BHA10560405

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA907557
Alternative Names	SH2 domain protein C1; SHC; SHC-transforming protein 1; SHCA; Src homology 2 domain-containing-transforming protein C1
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span Trampont P, et al. (2006) Mol Cell Biol; 26(23): 9035-9044.Patrussi L, et al. (2005) Oncogene; 24(13): 2218-2228van der Geer P, et al. (1996) Curr Biol 6(11): 1435-1444
Host	Rabbit
Immunogen	Peptide sequence around phosphorylation site of tyrosine 349 (H-Q-Y(p)-Y-N) derived from Human Shc1.
Raised In	Rabbit
Reactivity	Human, Mouse
Regulatory	RUO
Relevance	Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span Trampont P, et al. (2006) Mol Cell Biol; 26(23): 9035-9044. Patrussi L, et al. (2005) Oncogene; 24(13): 2218-2228 van der Geer P, et al. (1996) Curr Biol ; 6(11): 1435-1444
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of Shc1 only when phosphorylated at tyrosine 349.
Subcellular Location	Cytoplasm, SUBCELLULAR LOCATION: Isoform p46Shc: Mitochondrion matrix, Note=Localized to the mitochondria matrix, Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence, Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization, SUBCELLULAR LOCATION: Isoform p66Shc: Mitochondrion

Uniprot	P29353
Database Links	HGNC:10840 OMIM:600560 KEGG:hsa:6464 STRING:9606.ENSP00000401303 UniGene:Hs.433795
Function	Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis.
Pathway	Chemokine signaling pathway ErbB signaling pathway Estrogen signaling pathway Focal adhesion Ras signaling pathway Natural killer cell mediated cytotoxicity Insulin signaling pathway Phospholipase D signaling pathway Neurotrophin signaling pathway
Tissue Specificity	Widely expressed. Expressed in neural stem cells but absent in mature neurons.

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IF

Description

ELISA ; WB ; IF, WB ; ELISA ; IF

Dilution

WB	1:500-1:1000
IF	1:100-1:200

Tested Application

ELISA,WB,IF;WB:1:500-1:1000,IF:1:100-1:200

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10560405	CSB-PA907557	100ul	$360		Add to Cart

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