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Home / Primary Antibodies

Phospho-PXN (Tyr88) Antibody

SKU : BHA10560418

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA910129
Alternative Names	PAXI; PXN;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions Turner, C.E. (2000) J. Cell Sci. 113, 4139-4140Burridge, K. et al. (1992) J. Cell Biol. 119, 893-903.Bellis, S. L. et al. (1997) Biochem. J. 325, 375-381.
Host	Rabbit
Immunogen	Peptide sequence around phosphorylation site of tyrosine 88 (P-V-Y(p)-G-S) derived from Human Paxillin.
Raised In	Rabbit
Reactivity	Human
Regulatory	RUO
Relevance	An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions Turner, C.E. (2000) J. Cell Sci. 113, 4139-4140 Burridge, K. et al. (1992) J. Cell Biol. 119, 893-903. Bellis, S. L. et al. (1997) Biochem. J. 325, 375-381.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 88.
Subcellular Location	Cytoplasm, cytoskeleton, Cell junction, focal adhesion, Cytoplasm, cell cortex

Uniprot	P49023
Database Links	HGNC:9718 OMIM:602505 KEGG:hsa:5829 STRING:9606.ENSP00000228307 UniGene:Hs.446336
Function	Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Pathway	Chemokine signaling pathway VEGF signaling pathway Focal adhesion Regulation of actin cytoskeleton Leukocyte transendothelial migration
Protein Families	Paxillin family

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IF

Description

ELISA ; WB ; IF, WB ; ELISA ; IF

Dilution

WB	1:500-1:1000
IF	1:100-1:200

Tested Application

ELISA,WB,IF;WB:1:500-1:1000,IF:1:100-1:200

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10560418	CSB-PA910129	100ul	$360		Add to Cart

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