PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.Yue, C. et al. (1998) J. Biol. Chem. 273, 18023-18027. Margolis, B. et al. (1989) Cell 57, 1101-1107. Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225.
Host
Rabbit
Immunogen
Peptide sequence around phosphorylation site of tyrosine 771 (P-D-Y(p)-G-A) derived from Human PLC-g1.
Raised In
Rabbit
Reactivity
Human, Mouse, Rat
Regulatory
RUO
Relevance
PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
Yue, C. et al. (1998) J. Biol. Chem. 273, 18023-18027. Margolis, B. et al. (1989) Cell 57, 1101-1107. Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225.
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous level of PLC-gamma1 only when phosphorylated at tyrosine 771.
Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.
Pathway
Calcium signaling pathway ErbB signaling pathway HIF-1 signaling pathway VEGF signaling pathway NF-kappa B signaling pathway MAPK Erk pathway Ras signaling pathway Fc epsilon RI signaling pathway Fc gamma R-mediated phagocytosis Leukocyte transendothelial migration Natural killer cell mediated cytotoxicity T cell receptor signaling pathway Th1 and Th2 cell differentiation Th17 cell differentiation AGE-RAGE signaling pathway in diabetic complications Oxidative Phosphorylation Phosphatidylinositol signaling system Phospholipase D signaling pathway Rap1 signaling pathway Neurotrophin signaling pathway
Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.