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Home / Antibodies

Phospho-NCF1 (Ser304) Antibody

SKU : BHA10560174

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA910023
Alternative Names	NCF1; NCF-47K; P47 phox;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).Rodaway A.R.F., Mol. Cell. Biol. 10:5388-5396(1990).Gorlach A., J. Clin. Invest. 100:1907-1918(1997).Chanock S.J., Blood Cells Mol. Dis. 26:37-46(2000)
Host	Rabbit
Immunogen	Peptide sequence around phosphorylation site of Serine 304(R-S-S(p)-I-R) derived from Human Neutrophil Cytosol Factor 1.
Involvement In Disease	Granulomatous disease, chronic, cytochrome-b-positive 1, autosomal recessive (CGD1)
Raised In	Rabbit
Reactivity	Human
Regulatory	RUO
Relevance	NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). Rodaway A.R.F., Mol. Cell. Biol. 10:5388-5396(1990). Gorlach A., J. Clin. Invest. 100:1907-1918(1997). Chanock S.J., Blood Cells Mol. Dis. 26:37-46(2000)
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous levels of Neutrophil Cytosol Factor 1 only when phosphorylated at serine 304.
Subcellular Location	Cytoplasm, cytosol, Membrane, Peripheral membrane protein, Cytoplasmic side

Uniprot	P14598
Database Links	HGNC:7660 OMIM:233700 KEGG:hsa:653361 STRING:9606.ENSP00000289473 UniGene:Hs.647047
Function	NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Pathway	Chemokine signaling pathway Fc gamma R-mediated phagocytosis Leukocyte transendothelial migration Osteoclast differentiation
Tissue Specificity	Detected in peripheral blood monocytes and neutrophils (at protein level).

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB

Description

ELISA ; WB, WB ; ELISA

Dilution

WB	1:500-1:1000

Tested Application

ELISA,WB;WB:1:500-1:1000

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10560174	CSB-PA910023	100ul	$360		Add to Cart

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