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Home / Antibodies

Phospho-NBN (Ser343) Antibody

SKU : BHA10559920

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA069758
Alternative Names	NBN
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	Mutations in p95/NBS1 gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.Hsu HL, et al (2005) Oncogene; 24(31): 4956-64.Falck J, et al. (2005) Nature; 434(7033): 605-11.Buscemi G, et al. (2004) Oncogene; 23(46): 7691-700.Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A; 101(33): 12130-5.
Host	Rabbit
Immunogen	Peptide sequence around phosphorylation site of serine 343 (S-L-S(p)-Q-G) derived from Human p95/NBS1.
Involvement In Disease	Nijmegen breakage syndrome (NBS); Breast cancer (BC); Aplastic anemia (AA)
Raised In	Rabbit
Reactivity	Human
Regulatory	RUO
Relevance	Mutations in p95/NBS1 gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Hsu HL, et al (2005) Oncogene; 24(31): 4956-64. Falck J, et al. (2005) Nature; 434(7033): 605-11. Buscemi G, et al. (2004) Oncogene; 23(46): 7691-700. Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A; 101(33): 12130-5.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of p95/NBS1 only when phosphorylated at serine 343.
Subcellular Location	Nucleus, Nucleus, PML body, Chromosome, telomere

Uniprot	O60934
Database Links	HGNC:7652 OMIM:114480 KEGG:hsa:4683 STRING:9606.ENSP00000265433 UniGene:Hs.492208
Function	Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.
Pathway	Cellular senescence
Tissue Specificity	Ubiquitous. Expressed at high levels in testis.

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB

Description

ELISA ; WB, WB ; ELISA

Dilution

WB	1:500-1:1000

Tested Application

ELISA,WB;WB:1:500-1:1000

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10559920	CSB-PA069758	100ul	$360		Add to Cart

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