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Home / Antibodies

Phospho-MEF2A (Thr319) Antibody

SKU : BHA10559919

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA956856
Alternative Names	MEF2; Serum response factor-like protein 1;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domainShore, P. et al. (1995) Eur. J. Biochem. 229, 1-13Martin, J.F. et al. (1994) Mol. Cell. Biol. 14, 1647-1656Yu, Y.T. et al. (1992) Genes Dev. 6, 1783-1798Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30
Host	Rabbit
Immunogen	Peptide sequence around phosphorylation site of Thr319 (V-T-T(p)-P-S) derived from Human MEF2A.
Involvement In Disease	Coronary artery disease, autosomal dominant, 1 (ADCAD1)
Raised In	Rabbit
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Relevance	The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain Shore, P. et al. (1995) Eur. J. Biochem. 229, 1-13 Martin, J.F. et al. (1994) Mol. Cell. Biol. 14, 1647-1656 Yu, Y.T. et al. (1992) Genes Dev. 6, 1783-1798 Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 21-30
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of MEF2A only when phosphorylated at Thr319.
Subcellular Location	Nucleus

Uniprot	Q02078
Database Links	HGNC:6993 OMIM:600660 KEGG:hsa:4205 STRING:9606.ENSP00000346389 UniGene:Hs.268675
Function	Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter.
Pathway	Apelin signaling pathway cGMP-PKG signaling pathway
Protein Families	MEF2 family
Tissue Specificity	Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined.

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, IF

Description

ELISA ; IF, IF ; ELISA

Dilution

IF	1:100-1:200

Tested Application

ELISA,IF;IF:1:100-1:200

Datasheet
ELISA Protocol
Immunofluorescence (IF) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10559919	CSB-PA956856	100ul	$360		Add to Cart

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