DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Guettouche T, et al. (2005) BMC Biochem 6(1): 4 Wang X, et al. (2003) Mol Cell Biol 23(17): 6013-6026 Jolly C, et al. (1999) Proc Natl Acad Sci USA; 96(12): 6769-6774.
Host
Rabbit
Immunogen
Peptide sequence around phosphorylation site of serine 307 (P-Q-S(p)-P-R) derived from Human HSF1.
Raised In
Rabbit
Reactivity
Human
Regulatory
RUO
Relevance
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Guettouche T, et al. (2005) BMC Biochem ; 6(1): 4 Wang X, et al. (2003) Mol Cell Biol ; 23(17): 6013-6026 Jolly C, et al. (1999) Proc Natl Acad Sci USA; 96(12): 6769-6774.
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous level of HSF1 only when phosphorylated at serine 307.
Function as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage
Protein Families
HSF family
Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.