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Home / Antibodies

Phospho-CDC25B (Ser353) Antibody

SKU : BHA10560112

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA570157
Alternative Names	CDC25M2; MPIP2; Dual specificity phosphatase Cdc25B; CDC25HU2;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. Reboutier D, et al. (2012) J Cell Biol 197, 19-26 Lobjois V, et al. (2011) Biochem Biophys Res Commun 410, 87-90 Feng C, et al. (2007) Biol Reprod 77, 560-8
Host	Rabbit
Immunogen	Peptide sequence around phosphorylation site of serine 353(R-R-S(p)-V-T) derived from Human CDC25B .
Raised In	Rabbit
Reactivity	Human
Regulatory	RUO
Relevance	Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. Reboutier D, et al. (2012) J Cell Biol 197, 19-26 Lobjois V, et al. (2011) Biochem Biophys Res Commun 410, 87-90 Feng C, et al. (2007) Biol Reprod 77, 560-8
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of CDC25B only when phosphorylated at serine 353.
Subcellular Location	Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, spindle pole

Uniprot	P30305
Database Links	HGNC:1726 OMIM:116949 KEGG:hsa:994 STRING:9606.ENSP00000245960 UniGene:Hs.153752
Function	Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Pathway	MAPK signaling pathway Cell cycle
Protein Families	MPI phosphatase family

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB

Description

ELISA ; WB, WB ; ELISA

Dilution

WB	1:500-1:1000

Tested Application

ELISA,WB;WB:1:500-1:1000

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10560112	CSB-PA570157	100ul	$360		Add to Cart

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