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Home / Antibodies

Phospho-CDC25A (Ser178) Antibody

SKU : BHA10560111

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA065947
Alternative Names	CDC25M3; MPIP1; Dual specificity phosphatase Cdc25A; M-phase inducer phosphatase 1;
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.Chen MS, Ryan CE, Piwnica-Worms H (2003)Mol Cell Biol 23, 7488-97 Hassepass I, Voit R, Hoffmann I (2003) J Biol Chem 278, 29824-9 S rensen CS, et al. (2003) Cancer Cell 3, 247-58
Host	Rabbit
Immunogen	Peptide sequence around phosphorylation site of serine 178(Q-N-S(p)-A-P) derived from Human CDC25A .
Raised In	Rabbit
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Relevance	Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. Chen MS, Ryan CE, Piwnica-Worms H (2003)Mol Cell Biol 23, 7488-97 Hassepass I, Voit R, Hoffmann I (2003) J Biol Chem 278, 29824-9 S?rensen CS, et al. (2003) Cancer Cell 3, 247-58
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of CDC25A only when phosphorylated at serine 178.

Uniprot	P30304
Database Links	HGNC:1725 OMIM:116947 KEGG:hsa:993 STRING:9606.ENSP00000303706 UniGene:Hs.437705
Function	Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Pathway	Cell cycle Cellular senescence
Protein Families	MPI phosphatase family

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB

Description

ELISA ; WB, WB ; ELISA

Dilution

WB	1:500-1:1000

Tested Application

ELISA,WB;WB:1:500-1:1000

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10560111	CSB-PA065947	100ul	$360		Add to Cart

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