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Home / Antibodies

Phospho-CCND1 (Thr286) Antibody

SKU : BHA10560129

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA184970
Alternative Names	BCL-1 ; oncogene; CYL-1; PRAD1; PRAD1
Categories	Primary Antibodies ; Phospho Antibodies
Clonality	polyclonal
Description	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.Liou YC, et al. (2002) Proc Natl Acad Sci U S A 99, 1335-40 Tagliati F, et al. (2006) Endocrinology 147, 3530-8Zou Y, et al. (2004) J Biol Chem 279, 27790-8
Host	Rabbit
Immunogen	Peptide sequence around phosphorylation site of threonine 286 (A-C-T(p)-P-T) derived from Human Cyclin D1.
Involvement In Disease	Multiple myeloma (MM)
Raised In	Rabbit
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Relevance	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4. Liou YC, et al. (2002) Proc Natl Acad Sci U S A 99, 1335-40 Tagliati F, et al. (2006) Endocrinology 147, 3530-8 Zou Y, et al. (2004) J Biol Chem 279, 27790-8
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of Cyclin D1 only when phosphorylated at threonine 286.
Subcellular Location	Nucleus, Cytoplasm, Membrane

Uniprot	P24385
Database Links	HGNC:1582 OMIM:168461 KEGG:hsa:595 STRING:9606.ENSP00000227507 UniGene:Hs.523852
Function	Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
Pathway	Hedgehog signaling pathway Hippo signaling pathway Jak-STAT signaling pathway p53 signaling pathway PI3K-Akt signaling pathway Wnt signaling pathway Focal adhesion Cell cycle Cellular senescence AGE-RAGE signaling pathway in diabetic complications AMPK signaling Apelin signaling pathway FoxO signaling pathway
Protein Families	Cyclin family, Cyclin D subfamily

Buffer	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Purity	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB

Description

ELISA ; WB, WB ; ELISA

Dilution

WB	1:500-1:1000

Tested Application

ELISA,WB;WB:1:500-1:1000

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10560129	CSB-PA184970	100ul	$360		Add to Cart

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