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Home / Antibodies

Perlecan Antibody (Large) (V2601SAF)

SKU : BHA17124052

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NSJ Bioreagents

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Mfr.No.	V2601SAF-100UG
Clonality	Monoclonal (rat origin)
Description	This mAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan s strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Rat
Immunogen Region	A murine EHS laminin preparation was used as the immunogen for the anti-Perlecan antibody.
Isotype	Rat IgG2a, kappa
Species Reactivity	Human
Note	Optimal dilution of the anti-Perlecan antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P98160

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the anti-Perlecan antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124052	V2601SAF-100UG	100 ug	$499		Add to Cart

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