Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Rabbit
Immunogen Region
A portion of amino acids 413-552 was used as the immunogen for the PRF1 antibody.
Isotype
Rabbit IgG, kappa
Species Reactivity
Human
Note
Optimal dilution of the PRF1 antibody should be determined by the researcher.
Uniprot
P14222
Format
Purified
Purity
Protein A/G affinity
Storage
Aliquot the PRF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.