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Home / Antibodies

PCNA Antibody (V7004)

SKU : BHA17115225

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NSJ Bioreagents

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Mfr.No.	V7004, V7004-20UG, V7004-100UG
Antigen	PCNA
Clonality	Monoclonal
Description	Proliferating cell nuclear antigen is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. (UniProt)
Formulation	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Host	Mouse
Immunogen Region	Recombinant proliferating cell nuclear antigen was used as the immunogen for the PCNA antibody.
Isotype	IgG2a,kappa
Predicted Reactivity	Human
Reactivity	Human
Recombinant	No
Subcellular Location	Nuclear with some cytoplasmic

Uniprot	P12004
Clone No	PM441-1

Concentration	0.2 mg/ml
Format	Purified
Purification	Protein G affinity chromatography
Storage	Store the PCNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Applications	WB, IHC-P, FACS
Dilution	Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)

Product Manual	Data Sheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17115225-1	V7004-20UG	20ug	$219		Add to Cart
BHA17115225-2	V7004-100UG	100ug	$499		Add to Cart

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