Proliferating cell nuclear antigen (PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA/cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.
Formulation
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Host
Mouse
Immunogen Region
PCNA recombinant protein was used as the immunogen for this PCNA antibody.
Isotype
IgG2a,kappa
Predicted Reactivity
Human, Mouse, Rat, Chicken
Reactivity
Human, Mouse, Rat, Chicken
Recombinant
No
Gene Id
5111
Clone No
PC10
Format
Ascites
Purification
Unpurified ascites
Storage
After reconstitution, the PCNA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.