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Home / Antibodies

p62 Antibody / SQSTM1 (RQ5514)

SKU : BHA17110160

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NSJ Bioreagents

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Mfr.No.	RQ5514
Clonality	Monoclonal
Description	SQSTM1 (Sequestosome-1), also known as Ubiquitin-Binding Protein P62 or P62, is a protein that in humans is encoded by the SQSTM1 gene. The Src homology type 2 (SH2) domain is a highly conserved motif of about 100 amino acids which mediates protein-protein interactions by binding to phosphotyrosine.p56-lck, a T-cell-specific src family tyrosine kinase with an SH2 domain, is involved in T-cell signal transduction. The Inteational Radiation Hybrid Mapping Consortium mapped the p62 gene to chromosome 5q35. Park et al. (1995) found that the p56-lck SH2 domain binds to p62 at the ser59 of p62 only when that serine is phosphorylated. Joung et al. (1996) expressed epitope-tagged p62 in Hela cells and showed that the expressed protein bound to the lck SH2 domain and that this binding was dependent on the N-terminal 50 amino acids of p62 but not on the tyrosine residue in this region.
Formulation	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host	Mouse
Immunogen Region	Amino acids DEDGDLVAFSSDEELTMAMSYVKDDIFR were used as the immunogen for the SQSTM1 antibody.
Isotype	IgG2a
Predicted Reactivity	Human, Mouse, Rat
Reactivity	Human, Mouse, Rat
Recombinant	No
Subcellular Location	Cytoplasmic, membranous, nuclear

Uniprot	Q13501
Clone No	3H11

Buffer	Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Format	Antigen affinity purified
Purification	Affinity purified
Storage	After reconstitution, the SQSTM1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Applications	WB, IHC-P, IF, FACS
Dilution	Weste blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence: 2-4ug/ml

Product Manual	Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17110160	RQ5514	100 ug	$419		Add to Cart

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