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Home / Primary Antibodies

p53 Antibody / TP53 (V3513SAF)

SKU : BHA17125162

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NSJ Bioreagents

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Mfr.No.	V3513SAF-100UG
Clonality	Recombinant Rabbit Monoclonal
Description	Recognizes a 53 kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Rabbit
Immunogen Region	Recombinant human p53 protein was used as the immunogen for this recombinant p53 antibody.
Isotype	Rabbit IgG, kappa
Species Reactivity	Human
Note	The optimal dilution of the recombinant p53 antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P04637

Format	Purified
Purity	Protein A affinity chromatography
Storage	Store the recombinant p53 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	WB, IHC-P, FACS
Description	Weste blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Flow cytometry: 1-2ug/million cells

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17125162	V3513SAF-100UG	100 ug	$499		Add to Cart

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