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Home / Antibodies

p14ARF Antibody / CDKN2A (V3807SAF)

SKU : BHA17125630

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NSJ Bioreagents

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Mfr.No.	V3807SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	The progression of cells through the cell cycle is regulated by a family of proteins designated cyclin-dependent kinases (Cdks). Sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle. Multiple proteins are encoded by the tumor suppressor gene CDKN2A (MTS1/ p16INK4a) via translation of alternate reading frames, resulting in the production of the p19ARF protein in mice and the p14ARF protein in humans. p14ARF induces an increase in MDM2 and p21 levels and leads to cell cycle arrest in both G1 and G2/M. p14ARF is negatively regulated by p53 and is known to bind directly to MDM2. CDKN2A also encodes the mitotic protein p16, which binds to and inhibits the Cdk4/cyclin D complex.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Full-length recombinant human protein used as the immunogen for the p14ARF antibody.
Isotype	Mouse IgG2a, kappa
Species Reactivity	Human, Rat
Note	Optimal dilution of the p14ARF antibody should be determined by the researcher.

Uniprot	P42771

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the p14ARF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	WB, FACS, IF, IHC-P
Description	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17125630	V3807SAF-100UG	100 ug	$499		Add to Cart

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