CD134 is a type I integral membrane protein. This receptor is expressed on activated CD4+and CD8+T cells and B cells. The CD134 binds to CD134 ligand (CD252) to provide a costimulatory signal that is independent of CD28. CD134 is involved in coordinating CD4 T cell selection, migration and cytokine differentiation in T helper (Th)1 and Th2 cells. CD134 is also involved in the stimulation of T cells, T dependent humoral response and generation of optimal CD4+ T cell responses in vivo and in vitro. CD134 is expressed on activated CD4+ T lymphocytes, and its ligand, CD134L, is found preferentially on activated B cells. Engagement of CD134 with its ligand, CD134L, delivers a strong costimulatory signal to effector T cells.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Rat
Immunogen Region
Purified recombinant mouse OX40 antigen was used as the immunogen for the CD134 antibody.
Isotype
Rat IgG1, kappa
Species Reactivity
Mouse
Note
Optimal dilution of the antibody should be determined by the researcher.
Uniprot
P47741
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the CD134 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
FACS, IF
Description
This antibody does not block binding of OX40L,Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml