Recognizes a 53kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Rabbit
Immunogen Region
Recombinant human protein was used as the immunogen for the recombinant ODC1 antibody.
Isotype
Rabbit IgG, kappa
Species Reactivity
Human
Note
Optimal dilution of the recombinant ODC1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Uniprot
P11926
Format
Purified
Purity
Protein A affinity chromatography
Storage
Store the recombinant ODC1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
IHC-P, WB
Description
Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT,Western blot: 1-2ug/ml