This antibody recognizes a 53kDa protein, identified as the Oithine Decarboxylase. ODC-1 is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of oithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC-1 mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. Activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Recombinant human ODC-1 protein was used as the immunogen for this antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human, Mouse, Rat
Note
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the ODC-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, FACS, IF, IHC-P
Description
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Weste blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT (1) (2)