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Home / Primary Antibodies

Nuclear Antigen Antibody (V3107SAF)

SKU : BHA17124747

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NSJ Bioreagents

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Mfr.No.	V3107SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	This mAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This mAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled patte in normal and malignant cells and may be used to stain the nuclei of cells in fixed tissue sections.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Nuclei of HL60 cells were used as the immunogen for the Nuclear Marker antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human, Rat
Note	Optimal dilution of the Nuclear Marker antibody should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	Not Known

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Nuclear Marker antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IF, IHC-P
Description	Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124747	V3107SAF-100UG	100 ug	$499		Add to Cart

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