NADPH oxidase 4, also called Renal NADPH oxidase or RENOX, is an enzyme that in humans is encoded by the NOX4 gene, and a member of the NOX family of NADPH oxidases. Geiszt et al.(2000) stated that the nucleotide sequence of NOX4 matches that found in a genomic clone on chromosome 15. In a note added in proof, they stated that genomic clones assigned to chromosome 11 also contain sequence corresponding to NADPH Oxidase 4. By FISH, Shiose et al.(2001) mapped the gene to chromosome 11q14.2-q21. Geiszt et al.(2000) found that NIH 3T3 fibroblasts overexpressing the transfected protein showed increased production of superoxide and developed signs of cellular senescence. They suggested that NOX4, as a renal source of ROS, may fulfill the function of the putative oxygen sensor in the kidney. By biochemical analysis of endogenous renal NOX4, Shiose et al.(2001) determined that the enzyme can use either NADH or NADPH as an electron donor for superoxide production.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
An amino acid sequence from the C-terminus of mouse NADPH oxidase 4 (NRNNSYGTKFEYNKES) was used as the immunogen for this NOX4 antibody (100% rat homology).
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Human, Mouse, Rat
Recombinant
No
Uniprot
Q9JHI8
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the NOX4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, IHC-P, FACS
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells