The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. NOX4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. It is also expressed in fetal tissues, placenta, glioblastoma and vascular cells.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Human recombinant protein was used as the immunogen for this NOX4 antibody.
Isotype
Mouse IgG2b, kappa
Species Reactivity
Human
Note
Titering of the NOX4 antibody may be required for optimal performance.
Uniprot
Q9NPH5
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the NOX4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, ELISA
Description
ELISA (order BSA/sodium azide-free format for coating),Western blot: 0.5-1ug/ml