CD56/NCAM is a cell adhesion protein which is a member of the immunoglobulin superfamily. It is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. CD56 has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. (UniProt)
Formulation
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Host
Mouse
Immunogen Region
Recombinant human protein was used as the immunogen for the CD56 antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Titering of the CD56 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Uniprot
P13591
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the CD56 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
IHC-P
Description
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.