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Home / Antibodies

Multi Cytokeratin Antibody (V3065SAF)

SKU : BHA17124681

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NSJ Bioreagents

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Mfr.No.	V3065SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This mAb recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13) and 45kDa (CK18). This is a broad-spectrum mAb which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	A Keratin-enriched preparation from cultured human epithelial cells was used as the immunogen for the Multi Cytokeratin antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human, Rat
Note	Optimal dilution of the Multi Cytokeratin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	multi

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Multi Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124681	V3065SAF-100UG	100 ug	$499		Add to Cart

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