This mAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC). This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression patte opposite to MUC2. Together with a panel of antibodies, anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas. It has been found that treating the testing sample with Papain or mercaptoethanol will destroy the M1 epitope for the 2-11M1 antibody (Ref. 1; Cancer Research 46.8 (1986): 3983-3989.)
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
An M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient was used as the immunogen for the MUC5AC antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human, Mouse
Note
Optimal dilution of the MUC5AC antibody should be determined by the researcher.
Uniprot
P98088
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the MUC5AC antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).