The mucin antigen recognized by Mc-5 is a glycosylated molecule with a molecular mass of 400 kD. The sequence to which this antibody binds is Thr-Arg-Pro-Ala-Pro. Although EMA is primarily located in mammary gland epithelium, other normal epithelia (e.g., lung) will also react against EMA antibody. Staining, however, is the strongest in mammary epithelia. The combination of positive staining for keratin with negative EMA can be used to phenotype the above-mentioned epithelial tumors. Recognizes the mucin antigen that is present in most epithelial tissues (breast, colonic, bronchial, gastric, etc.) This antibody does not recognize myelopoietic, lymphopoietic, fibroblasts or sarcomatous cells.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Delipidated human milk fat globules were used as the immunogen for the MUC1 antibody. The sequence to which this antibody binds is Thr-Arg-Pro-Ala-Pro.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Optimal dilution of the MUC1 antibody should be determined by the researcher.
Uniprot
P15941
Format
Purified
Purity
Protein A/G affinity
Storage
Aliquot the MUC1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.